Search result for '98 '.
Viewing records 1 to 25 of 25 hits.
N8113
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Name: Ds Cre-lox line |
Price:
£11.00
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Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Photo
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Stock type: individual line
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Material type: seed
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Description transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; linked to m235; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8114
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Name: Ds Cre-lox line |
Price:
£11.00
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Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
|
|
Photo
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Stock type: individual line
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Material type: seed
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Description transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between 12s and g4560; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8115
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Name: Ds Cre-lox line |
Price:
£11.00
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Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Stock type: individual line
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Material type: seed
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Description transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between g4028 and m435; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8116
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Name: Ds Cre-lox line |
Price:
£11.00
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Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Stock type: individual line
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Material type: seed
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Description transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between m488 and g3786; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8117
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Name: Ds Cre-lox line |
Price:
£11.00
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Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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|
Stock type: individual line
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Material type: seed
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Description transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between m518a and pCITd23; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8118
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Name: Ds Cre-lox line |
Price:
£11.00
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Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Stock type: individual line
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Material type: seed
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Description transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron esistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin esistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; tightly linked to m600; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8119
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Name: Ds Cre-lox line |
Price:
£11.00
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Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Stock type: individual line
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Material type: seed
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; ightly linked to m506; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8120
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Name: Ds Cre-lox line |
Price:
£11.00
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Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Stock type: individual line
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Material type: seed
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; linked to g3845; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8121
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Photo
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Stock type: individual line
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Material type: seed
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between g4523 and m228; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8122
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Photo
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Stock type: individual line
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Material type: seed
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Description transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; linked to 12s; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8123
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
|
|
Photo
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Stock type: individual line
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Material type: seed
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Description transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between m488 and g3786; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8124
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Photo
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Stock type: individual line
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Material type: seed
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycine resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between g4552 and m532; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8125
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Photo
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Stock type: individual line
|
Material type: seed
|
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8126
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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|
Photo
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Stock type: individual line
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Material type: seed
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8127
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Photo
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Stock type: individual line
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Material type: seed
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; linked to m532; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8128
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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|
Photo
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Stock type: individual line
|
Material type: seed
|
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker
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N8129
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
|
|
Photo
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Stock type: individual line
|
Material type: seed
|
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8130
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
|
|
Photo
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Stock type: individual line
|
Material type: seed
|
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8131
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
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Stock type: individual line
|
Material type: seed
|
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8132
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
|
|
Photo
|
Stock type: individual line
|
Material type: seed
|
|
Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycine resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8133
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
|
|
Photo
|
Stock type: individual line
|
Material type: seed
|
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8134
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
|
|
Photo
|
Stock type: individual line
|
Material type: seed
|
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8135
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
|
|
Photo
|
Stock type: individual line
|
Material type: seed
|
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8136
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Name: Ds Cre-lox line |
Price:
£11.00
|
Donor
- The Plant Gene Expression Center Barbara Baker
- The Plant Gene Expression Center Cathy Corr
- The Plant Gene Expression Center Brian Osborne
|
|
Photo
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Stock type: individual line
|
Material type: seed
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Description Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
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N8137
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Name: Ds Cre-lox line |
Price:
£11.00
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Donor
- California Institute of Technology (Caltech) Joshua Levin
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Locus
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Photo
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Stock type: individual line
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Material type: seed
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Phenotype weak allele; probably lacks embryonic shoot apical meristem; bushy plant; produces aerial rosettes, reduced number of flowers per inflorescence; reduced numbers of petals, stamens, and carpels; female sterile; backcrossed once; maintained as heterozygote.
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