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Search result for '98 '. Viewing records 1 to 25 of 25 hits.



N8113 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; linked to m235; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8114 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between 12s and g4560; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8115 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Stock type: individual line
Material type: seed


Description
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between g4028 and m435; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8116 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Stock type: individual line
Material type: seed


Description
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between m488 and g3786; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8117 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Stock type: individual line
Material type: seed


Description
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between m518a and pCITd23; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8118 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Stock type: individual line
Material type: seed


Description
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron esistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin esistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; tightly linked to m600; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8119 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; ightly linked to m506; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8120 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; linked to g3845; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8121 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between g4523 and m228; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8122 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; linked to 12s; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8123 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between m488 and g3786; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8124 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycine resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; maps between g4552 and m532; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8125 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8126 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8127 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; linked to m532; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8128 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker
N8129 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the existing line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in W orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8130 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8131 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8132 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycine resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8133 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8134 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8135 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8136 Name: Ds Cre-lox line Price: £10.00
Donor
  • The Plant Gene Expression Center Barbara Baker
  • The Plant Gene Expression Center Cathy Corr
  • The Plant Gene Expression Center Brian Osborne
Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Description
Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.
N8137 Name: Ds Cre-lox line Price: £10.00
Donor
  • California Institute of Technology (Caltech) Joshua Levin
Locus Photo
photo of Arabidopsis plant
Stock type: individual line
Material type: seed


Phenotype
weak allele; probably lacks embryonic shoot apical meristem; bushy plant; produces aerial rosettes, reduced number of flowers per inflorescence; reduced numbers of petals, stamens, and carpels; female sterile; backcrossed once; maintained as heterozygote.