NASC

NASC ID: N564073

Name: SALK_064073

ABRC stock number: SALK_064073

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_064073.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G39720.1 (at chr 2 pos 16568434 (W/16568375-16568434) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 42, R 47, C 45 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO409458.

SALKseq_064073.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G10682=P355 and AT1G10690.1=UTR3 (at chr 1 pos 3549857 (W/3549788-3549857) on the TAIR10). The sequences' 1-70 bps mapped on the genome. Pools P 42, R 47, C 45 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO406117.

SALKseq_064073.101 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G52040.4=UTR3 and AT5G52050.1=P355 (at chr 5 pos 21138353 (C/21138353-21138390) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 44-70 bps onto the T-DNA. Pools P 42, R 47, C 45 N 41. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412811.

Donation Date: 2002-05-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_064073

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_064073C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G10682 AT1G10690 AT5G52040 AT5G52050 At2g39720

EMBL accession:

ED594389

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.