NASC

NASC ID: N571569

Name: SALK_071569

ABRC stock number: SALK_071569

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At4g22970. F7H19.150 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_071569.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT4G22970.2 (at chr 4 pos 12043675 (W/12043630-12043675) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 45-78 bps onto the T-DNA. Pools P 48, R 45, C 47 N 44. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO403246.

SALKseq_071569.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G26410.1=P355 and AT2G26420.1 (at chr 2 pos 11237702 (W/11237644-11237702) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 58-76 bps onto the T-DNA. Pools P 48, R 45, C 47 N 44. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393218.

SALKseq_071569.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G01450.4=P355 and AT2G01460.1 (at chr 2 pos 204040 (W/203990-204040) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 53-78 bps onto the T-DNA. Pools P 48, R 45, C 47 N 44. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO396557.

SALKseq_071569.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G62590.1 (at chr 3 pos 23148236 (C/23148236-23148314) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 48, R 45, C 47 N 44. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO399902.

Donation Date: 2002-01-07

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_071569

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_071569C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G01450 AT2G01460 AT2G26410 AT2G26420 AT3G62590 At4g22970

EMBL accession:

BH850604

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.