NASC Stock Detail Page
NASC ID: N578826
Name: SALK_078826
ABRC stock number: SALK_078826
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. July 2002 TAIR gene annotation - At3g19790. MMB12.25 R300-5'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_078826.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G19790.1 (at chr 3 pos 6875671 (W/6875594-6875671) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 64, R 61, C 61 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO430309. SALKseq_078826.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G05570.2=P355 and AT1G05575.1=UTR3 (at chr 1 pos 1660981 (W/1660918-1660981) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 64, R 61, C 61 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO417045. SALKseq_078826.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G64050.2=UTR3 and AT1G64060.1=P355 (at chr 1 pos 23766890 (W/23766813-23766890) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 64, R 61, C 61 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO420361. SALKseq_078826.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G62980.1 (at chr 3 pos 23274311 (C/23274311-23274367) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 64, R 61, C 61 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO423677. SALKseq_078826.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G09470.1 (at chr 3 pos 2914492 (W/2914438-2914492) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 64, R 61, C 61 N 64. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO426993.
Donation Date: 2002-01-07
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_078826
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N654735 (SALK_078826C).
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.