NASC

NASC ID: N8132

Name: Ds Cre-lox line

ABRC stock number: CS8132

Description: Transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycine resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements; stocks provided by C. Corr, B. Osborne, and B. Baker.

Donation Date: 1996-06-20

Donated by:

The Plant Gene Expression Center Barbara Baker The Plant Gene Expression Center Cathy Corr The Plant Gene Expression Center Brian Osborne

Donor Number: E52 (R)

Part of : Baker Ds Cre-lox

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Seed type: Transposon insertion

Mutagen: Ds Transposon Insertion

Background: No-0(N3081)

Transformation method: Agrobacterium transformation

References

Osborne, B.I., Wirtz, U. & Baker, B. 1995. A system for insertional mutagenesis and chromosomal rearrangement using the Ds transposon and Cre-lox. The Plant Journal 7(4):687-701.PubMed ID: 7742862.