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The European Arabidopsis Stock Centre

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NASC ID: N9987

Name: Saint Laurent Le Minier Nc5

ABRC stock number: CS9987

Description: Seeds were selected from a wild type accession (Nc5) from a Noccaea caerulescens Saint Laurent Le Minier population and selfed to S3 by single seed descent. Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively. Seeds were sown in plug trays (2 cm2 plugs) containing Levington M3 high nutrient peat-based compost (pH 5.3– 5.7) (Monro Group, Wisbech, Cambridgeshire, UK). Two week- old seedlings were then transplanted into 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Levington M3, sand (< 1 mm) and grit (1–3 mm) at a ratio of 2 : 1 : 1, respectively, v : v : v) (Monro Group). After 12 wk, plants were vernalized at 5°C with an 8 h photoperiod for 10 wk, before returning to GC for flowering, self fertilisation and infructescens. A single Zn solution was applied to all pots on a single occasion after 1 month of growth, supplying 55 mg Zn kg-1 compost.

Donation Date: 2011-01-11

Donated by:  University of Nottingham Martin Broadley
University of Nottingham Seosamh O'Lochlainn
University of Nottingham Neil Graham

Donor Number: Noccaea caerulescens

Part of : Noccaea caerulescens mutants/ecotypes

Stock type: individual line

Material type: seed

Comment: formerly known as Noccaea caerulescens

Status Price (£)
Currently unavailable £11.00

Germplasm Info

Genus: Thlaspi     Species: caerulescens

Seed type: Ecotype

Background: Thlaspi caerulescens

Segregation status: unknown

Growth requirement: Plants were grown under glasshouse conditions (GC) set to 16 h photoperiod using supplementary sodium lighting, with 22.3 and 13.3°C mean day and night temperatures, respectively, in 0.32 l pots (height 7.9 cm; diameter 9 cm) containing a compost mix (Lev

Phenotype

Single seed descent to S3 wild type lines, requiring vernalisation (5°C for 12 weeks) to initiate flowering. Fruiting between 7-9 months

Ecotype data

Location: Saint-Laurent-Le-Minier, Ganges

Habitat: Heavy-metal-enriched, Zn-Pb-Cd, lead/zinc mine spoil tailings

Country: FRANCE

Altitude Min: 100

Altitude Max: 500

Latitude Min: 43.93

Latitude Max: 43.93

Longitude Min: 3.67

Longitude Max: 3.67

References

Lochlainn, S. et al. 2011. Generation of non vernal-obligate, faster-cycling Noccaea caerulescens lines through fast neutron mutagenesis. New Phytologist 189(2):409-14.PubMed ID: 21058953.