NASC

NASC ID: N799855

Name: Mehlmer Set of Binary Plasmids

ABRC stock number: CS799855

Description: This collection contains a set of binary plasmids which targets the calcium sensor aequorin to different subcompartments of the plant cell. These constructs are aimed to generate transgenic Arabidopsis plants via agrobacterial transformation. The set includes constructs targeted to the cytosol, nucleus, inner surface of the plasma membrane, mitochondrial matrix, chloroplast stroma, as well as the inner and outer surface of the chloroplast envelope membranes.

NASC code	Localisation	Promoter	Plant selection	Paper (ID)
N799856	chloroplast	35S	Basta	pBIN-CHYA(B)
N799857	cytosol only	35S	Basta	pBIN-CYA(B)
N799858	IE chloroplast	35S	Basta	pBIN-IEYA(B)
N799859	mitochondria	35S	Basta	pBIN-MYA(B)
N799860	nucleus	35S	Basta	pBIN-NYA(B)
N799861	OE chloroplast	35S	Basta	pBIN-OEYA(B)
N799862	plasma membrane	35S	Basta	pBIN-PMYA(B)
N799863	chloroplast	Ubiquitin	Basta	pBINU-CHYA(B)
N799864	cytosol only	Ubiquitin	Basta	pBINU-CYA(B)
N799865	IE chloroplast	Ubiquitin	Basta	pBINU-IEYA(B)
N799866	mitochondria	Ubiquitin	Basta	pBINU-MYA(B)
N799867	nucleus	Ubiquitin	Basta	pBINU-NYA(B)
N799868	OE chloroplast	Ubiquitin	Basta	pBINU-OEYA(B)
N799869	plasma membrane	Ubiquitin	Basta	pBINU-PMYA(B)
N799870	cytosol and nucleus	Ubiquitin	Basta	pBINU-YA(B)
N799871	cytosol and nucleus	35S	Basta	pBIN-YA(B)
N799872	nucleus	Ubiquitin	Kanamycin	pBINU-NYA(K)
N799873	chloroplast	35S	Kanamycin	pBIN-CHYA(K)
N799874	cytosol only	35S	Kanamycin	pBIN-CYA(K)
N799875	IE chloroplast	35S	Kanamycin	pBIN-IEYA(K)
N799876	mitochondria	35S	Kanamycin	pBIN-MYA(K)
N799877	nucleus	35S	Kanamycin	pBIN-NYA(K)
N799878	OE chloroplast	35S	Kanamycin	pBIN-OEYA(K)
N799879	plasma membrane	35S	Kanamycin	pBIN-PMYA(K)
N799880	chloroplast	Ubiquitin	Kanamycin	pBINU-CHYA(K)
N799881	cytosol only	Ubiquitin	Kanamycin	pBINU-CYA(K)
N799882	IE chloroplast	Ubiquitin	Kanamycin	pBINU-IEYA(K)
N799883	mitochondria	Ubiquitin	Kanamycin	pBINU-MYA(K)
N799884	OE chloroplast	Ubiquitin	Kanamycin	pBINU-OEYA(K)
N799885	plasma membrane	Ubiquitin	Kanamycin	pBINU-PMYA(K)
N799886	cytosol and nucleus	Ubiquitin	Kanamycin	pBINU-YA(K)
N799887	cytosol and nucleus	35S	Kanamycin	pBIN-YA(K)

Donation Date: 2013-06-21

Donated by:

Ludwig-Maximilians-Universität München (LMU) Norbert Mehlmer

Stock type: set (View set contents)

Material type: plasmid dna

Status	Price (£)
Available	£96.00

Germplasm Info

References

Mehlmer, N. et al. 2012. A toolset of aequorin expression vectors for in planta studies of subcellular calcium concentrations in Arabidopsis thaliana. Journal of experimental botany 63(4):1751-61. PubMed ID: 22213817.